Column for reversed phase
Develosil ODS
Develosil ODS‐K
Develosil ODS‐N
Develosil ODS‐P
Develosil ODS‐A
Develosil ODS‐T
The origin of Develosil ODS Column!!
Six kinds of ODS columns released it approximately 30 years ago. “Developing new ideas based on study of the past.”
All began in here.
Physical properties of Develosil OD S Series
|
Column name |
Ligand |
Carbon |
End Capping |
Surface area |
Pore diameter |
Pore volume |
Range of pH |
|
ODS |
Octadecyl (Monfunctional) |
20% |
Yes (Single) |
350m2/g |
12nm |
1.05mL/g |
pH2-7.5 |
|
ODS-K |
Octadecyl (Monfunctional) |
19% |
Yes (Single) |
||||
|
ODS-N |
Octadecyl (Monfunctional) |
17% |
Yes (Single) |
||||
|
ODS-P |
Octadecyl (Monfunctional) |
11% |
Yes (Single) |
||||
|
ODS-T |
Octadecyl (Trifunctional) |
20% |
Yes (Single) |
||||
|
ODS-A |
Octadecyl (Trifunctional) |
19% |
No |
The width of the selection to its maximum !!
Six kinds of ODS is used to a purpose. As for it, the choice of the column which is most suitable by the action of hydrophobicity and a left silanol group is possible.
Comparison of standard chromatograms on the same condition
When the column is selected, it is necessary to know the performance of the column enough. I will compare it from hydrogen bonding, hydrophobicity, plane recognition characteristics what kind of performance six kinds of columns have here.
Comparison of six‐kind ODS column hydrogen bonding
Comparison of six‐kind ODS column Hydrophobicity
It is known that the residual silanol group affects the shape of the peak in the analysis of the basic compound greatly. To evade this, the carbon content such as ODS, ODS‐k, and ODS‐T selects the one that the end cap processing is done high. Hydrophobicity is a high column in ODS, ODS‐K, and ODS‐T for this. It can be said that it is a standard so‐called column.
Plane recognition‐related comparison
Comparison between ODS‐5 and ODS‐T‐5
It is set to ODS‐5 and ODS‐T‐5 with the difference between monofunctional type ODS or polymeric type ODS.
The separation level α;
ODS‐5 α= 1.49
ODS‐T‐5 α=1.74
A difference comes out by ODS reagent to couple.
Comparison between ODS‐T‐5 and ODS‐A‐5
It is the difference in the existence of an end cap ODS‐T‐5 and ODS‐A‐5.
The separation level α;
ODS‐T‐5 α=1.74
ODS‐A‐5 α=2.32
The effect of the end cap understands that there are more effects than the form of the ODS reagent.
Analysis example of the preservative
An analysis example of the preservative using six kinds of ODS. Retention tends to rise in ODS, ODS‐K, ODS‐N so that carbon content is low. When a high place does shortening at time because all separates all columns well, ODS‐P and ODS‐T, ODS‐A are suitable.
Analysis example of the preservative
Analysis example of phenytoin
Analysis example of phenytoin by six kinds of ODS columns.
Phenytoin is one kind of the antiepileptic drug of the hydantoin system. I enable simple analysis in a mobile phase only for acetonitrile and water.
Analysis example of phenytoin
The example of analysis of a basic compound
An analysis example of the basic compound by six kinds of ODS columns. I use pyridine for a object for comparison. The basic compound is affected by the silanol group, and retention changes. While carbon content is approximately equal, a big difference comes out to ODS‐T and ODS‐A‐5 by having end cap or not.
The example of analysis of a basic compound
