The Develosil HSR C18 Peptide was specifically designed for your analysis of amino acids, peptides, and nucleic acids. This column adopts the HSR series’s refined silica gel base material and is Develosil’s first column where functional group density is controlled. The Develosil HSR C18’s newly constructed bonding technology greatly contributes to durability and smoother method development.
Develosil HSR C18 Peptide Specifications
| Ligand | Carbon | End Capping | Surface Area | Pore Diameter | pH range | Temperature Range | Particle Size |
|---|---|---|---|---|---|---|---|
| Octadecyl Silica (ODS) |
17% | Yes | 300 m²/g | 14 nm | pH 1-10 | 5°-80°C | 2.5 µm , 3 µm, 5 µm |
Featured Applications
Separation Comparison of Peptides
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)Develosil HSR C18 Peptide, 5 µm (4.6 x 150 mm)
Other company 300 Å C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A) Water 0.1% TFA B) Acetonitrile +0.1% TFA |
| Gradient: | B) 0%-50%(0-30 min), 50%-0%(30.1-40 min), 0%(40.0min-) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 50°C |
| Detection: | UV at 210 nm |
| Sample: | 1. Gly-Tyr 2. Val-Tyr-Val 3. Met-Enkephalin 4. Angiotensin II 5. Leu-Enkephalin 6. γ-Endorphin 7. ß-Endorphin 8. Insultin |
| Injection Volume: | 20.0 µL |
| System | Thermofisher SCIENTIFIC Ultimate 3000 |
| Mixer Volume | 150 µL |
Separation Comparison of dNTP
| HPLC Analytical Conditions: | |
|---|---|
| Column: | C18 Column, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | 25mM Phosphate Buffer. pH=7.0 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 254 nm |
| Sample: | 1. dCTP (0.13 mM) 2. dTTP (0.13 mM) 3. dGTP (0.13 mM) 4. dATP (0.13 mM) |
| Injection Volume: | 2.0 µL |