The Develosil HSR C18 is your go-to C18 column. It supports all HPLC analyses with high selectivity and high separation performance. This ODS column uses our own-production Si gel that has a large surface area to give you overwhelming retention capacity for your HPLC separations. The Develosil HSR C18 is subjected to a special end cap treatment that offers good peak shape in acidic, basic, or neutral conditions.
Develosil HSR C18 Specifications
| Ligand | Carbon | End Capping | Surface Area | Pore Diameter | Pore Volume | Range of pH | Column Withstand Pressure |
|---|---|---|---|---|---|---|---|
| ODS/C18 (Polymeric) | 22% | Yes | 450 m²/g | 80 Å | 1.15 ml/g | pH 1-10 | 25 MPa |
Featured Applications
Separation Comparison of Pesticides
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil HSR C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | Acetonitrile/25mM NH₄H₂PO₄, pH=2.0=45/55 |
| Flow Rate: | 1.0 mL/min |
| Detection: | UV at 210 nm |
| Sample: | 1. Asulam (0.98 mg/mL) 2. Thiuram (1.12 mg/mL) 3. Triclopyr (1.01 mg/mL) 4. Flazasulfuron (0.97 mg/mL) 5. MCPP (0.97 mg/mL) 6. Halosulfuron-methyl (1.06 mg/mL) |
| Injection Volume: | 2.0 µL |
Capsaicin in Food
| HPLC Analytical Conditions: | |
|---|---|
| Column: | Develosil HSR C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | Acetonitrile/0.1% Phosphoric Acid=60/40 |
| Flow Rate: | 1.0 mL/min |
| Detection: | UV at 280 nm |
| Sample: | 1. Capsaicin (0.59 mg/mL) 2. Dihydrocapsaicin (0.55 mg/mL) |
| Injection Volume: | Food sample: 10 µL Standard: 2.0 µL |
Tricyclic Antidepressants
HPLC Analytical Conditions: |
|
|---|---|
| Column: | Develosil HSR C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | (MeOH ver.) MeOH/25mM (NH₄)₂HPO₄, pH7.0=80/20 (Acetonitrile ver.) ACN/25mM (NH₄)₂HPO₄, pH7.0=60/40 |
| Flow Rate: | 1.0 mL/min |
| Detection: | UV at 280 nm |
| Sample: | 1.Desipramine HCl (64ug/mL) 2.Nortriptyline HCl (52ug/mL) 3.Imipramine HCl (54ug/mL) 4.Amitriptyline HCl (53ug/mL) |
| Injection Volume: | 2.0 µL |