The Develosil AQ HSR C18 and Develosil HSR C1 help you separate high polarity compounds on a reverse phase column. The Develosil HSR AQ C18 and Develosil HSR C1 gives you maximum performance even in 100% aqueous mobile phase conditions.
As being part of the HSR series, these columns are subjected to original end cap technology to maximize peak shape in acidic or basic conditions.
Applications
| HPLC Analytical Conditions: | |
|---|---|
| Column: | 1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
2. Develosil HSR C1, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | Methanol/25mM NH₄H₂PO₄ (pH=2.0)=25/75 |
| Gradient: | B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 280 nm |
| Sample: | 1. (-)-Epigallocatechin (0.25mg/mL) 2. (-)-Epigallocatechin gallate (0.30mg/mL) 3. Caffeine 4. (-)-Epicatechin (0.25mg/mL) 5. (-)-Epicatechin gallate (0.30mg/mL) |
| Injection Volume: | 2.0 µL |
| HPLC Analytical Conditions: | |
|---|---|
| Column: | 1. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm)
2. Develosil HSR C1, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | Methanol/25mM NH₄H₂PO₄ (pH=2.0)=25/75 |
| Gradient: | B) 5%(0-2min), 5%-50%(2-7min), 50%(7-10min), 5%(10.1min-) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 40°C |
| Detection: | UV at 280 nm |
| Sample: | 1. (-)-Epigallocatechin (0.25mg/mL) 2. (-)-Epigallocatechin gallate (0.30mg/mL) 3. Caffeine 4. (-)-Epicatechin (0.25mg/mL) 5. (-)-Epicatechin gallate (0.30mg/mL) |
| Injection Volume: | 2.0 µL |
HPLC Analytical Conditions: |
|
|---|---|
| Column: | 1. Develosil HSR C1, 5 µm (4.6 x 150 mm) 2. Develosil HSR AQ C18, 5 µm (4.6 x 150 mm) |
| Mobile Phase: | A. 25 mM NaCl (pH 3.0) B) Acetonitrile |
| Flow Rate: | 1.0 mL/min |
| Gradient: | See more data here. |
| Temperature | 30°C |
| Sample: | 1.Gly-Tyr (0.05mg/mL) 2.Val-Tyr-Val (0.05mg/mL) 3.Met-Enkephalin (0.05mg/mL) 4.Angiotensin II (0.05mg/mL) 5.Leu-Enkephalin (0.05mg/mL) |
| Injection Volume: | 10.0 µL |