Develosil 100Diol and 300Diol are the columns developed for gel filtration chromatography. The size exclusion chromatography of water soluble polymers, such as hundreds of thousands of protein, enzymes, etc., is possible from the molecular weight 10,000. packing material type: For the compounds of low molecular weight ⇒ 100 Diol For the amounts of macromolecules ⇒ 300 Diol That is, since there are two kinds, low molecular weight and the amount of macromolecules, a column can be chosen according to the molecular weight of the purpose reagent.
Physical properties of Develosil 100Diol and 300Diol :
Protein and a polyethylene glycol calibration curve
The calibration curve of exclusion limit protein
The calibration curve of polyethylene glycol
Size exclusion limit molecular weight of Develosil 100Diol and 300Diol
Please reference this exclusion limit molecular weight as a standard.
Applications
Analysis of a person blood serum
Column: Develosil 300 Diol-5 8.0x300mm
Develosil 100 Diol-5 8.0x300mm
Eluate: 0.1M phosphate buffer + 0.2M NaCl (pH6.8)
Flow reate: 0.5mL/min
Detection: UV280nm
Analysis of albumen
Column: Develosil 300 Diol-5 8.0x300mm
Develosil 100 Diol-5 8.0x300mm
Eluate: 0.1M phosphate buffer + 0.2M NaCl (pH6.8)
Flow reate: 0.5mL/min
Detection: UV280nm
Analysis of standard protein
Column: Develosil 300 Diol-5 8.0x300mm
Eluate: 0.1M phosphate buffer + 0.2M NaCl (pH6.8)
Flow reate: 0.5mL/min
Detection: UV280nm
Bibliography
【Measurement of the uncombined type concentration of drug in the blood by the HPFA method using Develosil 100Diol-5 column】
Determination of Unbound Concentration of Hydrophobic Drugs in Albumin Solutions by High-Performance Frontal Analysis Using a Diol-Silica Column .
Akimasa Shibukawa , Chikako Nakao , Takeshi Sawada , Akira Terakita , Noritsugu Morokoshi , Terumichi Nakagawa.
J.Pharm.Sci. , 83(1994) 868-873
Table 1-HPLC Conditions for the Determination of the Unbound Diclofenac Concentration
HPFA
Off-Line Reversed-Phase HPLC
